Multiple interaction modes between saccharin and sweet taste receptors determine a species‐dependent response to saccharin. Issue 2 (31st December 2021)

• Record Type: Journal Article
• Authors: Zhao, Xiangzhong; Liu, Meng; Cui, Meng; Liu, Bo
• Is Part Of: FEBS open bio. Volume 12:Issue 2(2022)
• Page Start: 494
• Page End: 499
• Publication Date: 2021-12-31
• Subjects: allosteric binding site -- inhibition, interaction modes -- saccharin -- species‐dependent sweet taste -- sweet taste receptor; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13355
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Saccharin is a commonly used artificial sweetener that exhibits both sweetening and sweet inhibition activities. The species‐dependent response towards saccharin and the interaction between saccharin and the sweet taste receptor T1R2/T1R3 remain elusive. In this study we used mismatched chimeras of T1R2 and T1R3 and calcium mobilization functional analysis to reveal a detailed species‐dependent response towards saccharin of human, squirrel monkey, and mouse sweet taste receptors. Our findings, combined with previous results by others, suggest multiple and complex interaction modes between saccharin and the sweet taste receptor, which are helpful guidelines for effective modulation of the sweet taste by sweetener/sweet inhibitors. Abstract : Saccharin exhibits both sweetening and sweet inhibition activities. Besides the orthosteric binding site in T1R2 for activating the human sweet taste receptor T1R2/T1R3, it appears that there are at least two allosteric inhibitory sites located in T1R2 and T1R3 respectively to inhibit the receptor activity, suggesting multiple and complex interaction modes between saccharin and the heterodimeric sweet taste receptor T1R2/T1R3.