Inhibitory effects of a low-molecular-weight sulfated fucose-containing saccharide on α-amylase and α-glucosidase prepared from ascophyllan. Issue 3 (12th January 2022)

• Record Type: Journal Article
• Authors: Zhan, Hui; Yu, Gang; Zheng, Mingjing; Zhu, Yanbing; Ni, Hui; Oda, Tatsuya; Jiang, Zedong
• Is Part Of: Food & function. Volume 13:Issue 3(2022)
• Page Start: 1119
• Page End: 1132
• Publication Date: 2022-01-12
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo03331j
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : A low-molecular-weight sulfated fucose-containing saccharide with potent inhibition on α-glucosidase but mild inhibition on α-amylase was prepared from alginate lyase digested ascophyllan, having desirable characteristics as an anti-diabetic agent. Abstract : To find natural and safe anti-diabetic foods or potential drugs, low-molecular-weight saccharide fragments LMWAs-H ( M w 33.48 kDa) and LMWAs-L ( M w 6.71 kDa) from the sulfated polysaccharide ascophyllan of Ascophyllum nodosum using alginate lyase (EC 4.2.2.3) were investigated. The results revealed that LMWAs-H possessed potent inhibition activity against α-glucosidase or α-amylase in a concentration-dependent manner, which were higher than native ascophyllan or LMWAs-L. LMWAs-H exhibited a stronger inhibitory activity against α-glucosidase than α-amylase because it differently affects the conformational structures of these enzymes. Structural analysis revealed LMWAs-H to be →4)-α-l -Fuc p -(1 → 4)-α-l -Fuc p -(1 → 3)-β-d -Xyl p -(1 → 3)-α-l -Fuc p 4S(1→ as main chain, and T -α-d -Glc p -(1→ and →3)-β-d -Man p Ared residues were attached to the ends of main chain as non-reducing- and reducing-end residues, respectively. The 4-deoxy-l - erythro -hex-4-enuronosyluronate linked the O-4 position of →3, 4)-β-d -ManpAred residue as side branches. Our results suggest that LMWAs-H is the main active structural motif responsible for the enzymes-inhibiting activities, which is probably derived from the fucose-containing branches of ascophyllan. Our findings reveal that the strong inhibition of LMWAs-H on α-glucosidase but mild inhibition on α-amylase is highly related to its structural properties, suggesting its desirable characteristics as an anti-diabetic agent.