Hypoglycemic effects of Rhodiola crenulata (HK. f. et. Thoms) H. Ohba in vitro and in vivo and its ingredient identification by UPLC-triple-TOF/MS. Issue 3 (26th January 2022)

• Record Type: Journal Article
• Authors: Yue, Huilan; Wang, Luya; Jiang, Sirong; Banma, Cailang; Jia, Wenjing; Tao, Yanduo; Zhao, Xiaohui
• Is Part Of: Food & function. Volume 13:Issue 3(2022)
• Page Start: 1659
• Page End: 1667
• Publication Date: 2022-01-26
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo03436g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : The anti-hyperglycaemic effects of Rhodiola crenulata (HK. f. et. Thoms) H. Ohba (RC) was firstly demonstrated in vivo and in vitro, and 40 ingredients in RC were detected and identified by UPLC-Triple-TOF-MS. Abstract : Rhodiola crenulata (HK. f. et. Thoms) H. Ohba (RC), mainly distributed in the highly cold region of China, has long been used as a medicine/healthy food for eliminating fatigue and increasing blood circulation. This study aimed to evaluate the inhibitory effects of the RCRS extract on α-amylase and α-glucosidase (sucrase and maltase) in vitro and in vivo, and tentatively analyze and identify its chemical ingredients using UPLC-Triple-TOF/MS. The Rhodiola crenulata RCRS extract had strong inhibitory activities against α-amylase, sucrase and maltase with an IC50 of 0.031 mg mL −1, 0.142 mg mL −1 and 0.214 mg mL −1, respectively. Furthermore, the RCRS extract could significantly decrease the postprandial blood glucose (PBG) level of normal mice in a starch tolerance test, and reduce the PBG levels of diabetic mice in a starch/maltose/sucrose tolerance test. UHPLC-Triple-TOF-MS/MS analysis indicated that hydroxybenzoic acids, hydroxycinnamic acids, alcohol glycosides, flavonols and their derivatives were the main active ingredients in the RCRS extract. The results demonstrate that the RCRS extract of Rhodiola crenulata could be employed as a healthy food or medicine for controlling postprandial blood glucose levels.