Separation of antibody IgG201 by an aqueous two-phase system with recyclable pH-responsive polymers. (February 2022)

• Record Type: Journal Article
• Authors: Chen, Xi; Guo, Yibo; Yang, Ting; Wan, Junfen; Cao, Xuejun
• Is Part Of: Process biochemistry. Volume 113(2022)
• Page Start: 125
• Page End: 133
• Publication Date: 2022-02
• Subjects: Monoclonal antibody purification -- Aqueous two-phase system -- Recyclable pH-response polymers; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.12.017
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Recycling aqueous two-phase system with pH-response polymers PA and PB. pH responsive ATPS was applied to separate therapeutics monoclonal antibodies. ATPS separation is a spontaneous endothermic reaction. 92.2 % purity without bioactivity loss were achieved after ATPS partition. Abstract: Since their clinical application success, therapeutic monoclonal antibodies (mAbs) have been playing important roles in the treatment of many diseases. A significant obstacle to reducing antibody drug costs is reducing the use of expensive materials in the purification manufacturing process. New technologies, such as aqueous two-phase systems (ATPSs), are an option to solve this problem. In this study, a recyclable aqueous two-phase system was developed. Two pH-responsive polymers were synthesized and characterized. The phase-forming polymers and salt concentrations, system pH, and temperature were studied. Finally, under conditions of 6% PMDB3.36 with 20 mmol/L sodium acetate and 30 mmol/L Na2 SO4 at pH 5.5, the recovery of target monoclonal antibody IgG201 was 94.4 % and partition coefficient was 8.7. The purity was 92.2 %, as determined bysize exclusion chromatography (SEC). The pH-sensitive polymers in this ATPS can be recycled near their isoelectric point.