Techno-functional properties and immunomodulatory potential of exopolysaccharide from Lactiplantibacillus plantarum MM89 isolated from human breast milk. (30th May 2022)

• Record Type: Journal Article
• Authors: Rajoka, Muhammad Shahid Riaz; Mehwish, Hafiza Mahreen; Kitazawa, Haruki; Barba, Francisco J.; Berthelot, Laureline; Umair, Muhammad; Zhu, Qinchang; He, Zhendan; Zhao, Liqing
• Is Part Of: Food chemistry. Volume 377(2022)
• Publication Date: 2022-05-30
• Subjects: Lactiplantibacillus plantarum -- Human breast milk, exopolysaccharide -- Immunomodulatory activity -- Techno-functional properties; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131954
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: An exopolysaccharide was obtained from Lactiplantibacillus plantarum . The exopolysaccharide exhibited excellent techno-functional properties. The exopolysaccharide showed potent immunomodulatory activity in-vitro . The exopolysaccharide increased immunomodulatory activity in immunosuppressed mice. Abstract: An exopolysaccharide, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum MM89. It was comprised of glucose and mannose molecules with an average molecular weight of 138 kDa. FTIR and NMR spectra showed that MM89-EPS had characteristic polysaccharide functional groups. MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. To summarize, our results indicate that MM89-EPS can efficiently enhance the immunostimulatory activity of immune cells and an immunosuppressed mouse model. Hence, MM89-EPS may be use as a potential source of immunomodulatory agent in various food products.