A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein. (1st June 2022)

• Record Type: Journal Article
• Authors: Wang, Jinbin; Yang, Qianwen; Liu, Hua; Chen, Yifan; Jiang, Wei; Wang, Yu; Zeng, Haijuan
• Is Part Of: Food chemistry. Volume 378(2022)
• Publication Date: 2022-06-01
• Subjects: Lateral flow immunoassays -- Thionine-labeling -- CP4-EPSPS protein -- Genetically modified crops; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132112
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A nanomaterial-free lateral flow immunoassay was developed. The thionine was used as a reporter. The method was used to screen transgenic crops containing the CP4-EPSPS protein. The vLODs were 0.05%, 0.1% and 0.1% for RRS, H7-1 and MON88913. The test strip was fast, specific and sensitive. Abstract: Nanomaterial-based lateral flow immunoassays (LFIAs) have been widely used for the on-site detection of genetically modified components. However, the practical applications are often limited by the complex matrix, such as in red samples. In this study, a thionine (Thi) labeling-based LFIA was developed for the first time to detect CP4-EPSPS protein. The optimal labeling concentration of Thi was 0.5 mg/mL, and the antibody could be rapidly coupled to Thi in 10 min. The visual limit of detection (vLOD) levels for transgenic soybean, sugar beet, and cotton containing the CP4-EPSPS protein reached 0.05%, 0.1%, and 0.1%, respectively, and had no interference from other proteins. After storage at 4 °C for three months, the LFIA sensitivity remained unchanged and showed good stability. This method could be used to screen and detect a variety of transgenic crops containing the CP4-EPSPS protein, and the results were consistent with the current standard assay. This study pioneered the development of an immunochromatographic method using Thi as a marker and applied it to the detection of the CP4-EPSPS protein in herbicide-tolerant transgenic crops. This provides a new method for the rapid immunoassay of Thi as a dye and has good prospects for practical application.