HPLC-potentiometric method for determination of biogenic amines in alcoholic beverages: A reliable approach for food quality control. (15th March 2022)

• Record Type: Journal Article
• Authors: Gil, Renato L.; Amorim, Célia G.; Montenegro, Maria C.B.S.M.; Araújo, Alberto N.
• Is Part Of: Food chemistry. Volume 372(2022)
• Publication Date: 2022-03-15
• Subjects: Alcoholic beverages -- Biogenic amines -- Liquid chromatography -- Potentiometry -- Ion-selective electrodes; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131288
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: HPLC-potentiometric method for quantification of ten underivatized biogenic amines. Improved selectivity and sensitivity in a greener chemistry context. Detection limits ranged from 9.3 to 60.5 µg L −1 . The proposed methodology was successfully applied in alcoholic beverages. Simple, user-friendly and reliable method for food quality control. Abstract: Determination of ten biogenic amines in alcoholic beverages by HPLC coupled to a potentiometric detector for food quality control is herein presented. Biogenic amines were separated by ion-pair chromatography on a C18 column using a gradient mobile phase of acetic acid, acetonitrile, and butane-sulfonic acid. Detection was accomplished by a miniaturized amine-selective electrode. The method was validated following ICH and Eurachem guidelines. Linear regression models provided R 2 values from 0.9870 ± 0.0019 to 0.9991 ± 0.0014 for tyramine and cadaverine, respectively. Detection and quantification limits depend on the molecular weight of BAs, ranging from 9.3 to 60.5 and from 31.1 to 202.3 µg L −1 for methylamine and spermine, respectively. Repeatability and intermediate precision showed RSD values lower than 5.8 and 8.3%, respectively. Accuracy of assays yielded recovery values from 86.4 to 109.9%. The biogenic amines content in red wine, white wine, and beer samples were 7.54, 5.24, and 4.58 mg L -1, respectively.