Whole‐genome sequencing of extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae from retail raw milk. (16th November 2021)

• Record Type: Journal Article
• Authors: Aslantaş, Özkan; Yılmaz, Ebru Şebnem; Büyükaltay, Kaan; Kocabağ, Hamide Deniz
• Is Part Of: International journal of dairy technology. Volume 75:Number 1(2022)
• Page Start: 77
• Page End: 82
• Publication Date: 2021-11-16
• Subjects: Enterobacteriaceae -- ESBL -- pAmpC -- Raw milk -- Whole‐genome sequencing; Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12828
• Languages: English
• ISSNs: 1364-727X

Abstract

Abstract : The contaminated products of food‐producing animals have been proposed to lead the transmission of resistant bacteria to consumers. Extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae, which were isolated from raw milk samples, were characterised using whole‐genome sequencing in this study. Three confirmed ESBL and one plasmid‐mediated AmpC β‐lactamase (pAmpC)‐producing isolate were obtained. All of the ESBL‐producing isolates showed a multidrug‐resistant (MDR) phenotype, but the pAmpC‐producing isolate was only resistant to β‐lactams. This study showed that raw milk can contain MDR bacteria that have several resistance determinants and highlighted the necessity of efficiently and routinely screening the products of food‐producing animals. Abstract : Legend of the graphical abstract: Molecular characterization of ESBL‐ and pAmpC producing Enterobacteriaceae in retail raw milk.