Highly selective monoclonal antibody-based lateral flow immunoassay for visual and sensitive determination of conazole fungicides propiconazole in vegetables. Issue 1 (2nd January 2022)

• Record Type: Journal Article
• Authors: Xu, Ze-Ke; Meng, Jing-Nan; Lei, Yi; Yang, Xing-Xing; Yan, Yi-Yong; Liu, Hai-Hong; Lei, Hong-Tao; Wang, Ting-Cai; Shen, Xing; Xu, Zhen-Lin
• Is Part Of: Food additives & contaminants. Volume 39:Issue 1(2022)
• Page Start: 92
• Page End: 104
• Publication Date: 2022-01-02
• Subjects: Propiconazole -- monoclonal antibody -- immunochromatographic test strip -- rapid detection; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2021.1976423
• Languages: English
• ISSNs: 1944-0049

Abstract

ABSTRACT: The conazole fungicide propiconazole is frequently found in vegetables although usage is not allowed. To overcome the high-cost and time-consuming labour requirements of instrumental methods, we developed a simple and visual lateral flow immunoassay for the sensitive determination of propiconazole. A hapten was carefully designed to raise a monoclonal antibody against propiconazole. Bal b/c mice were immunised with the hapten-carrier protein conjugate and a specific monoclonal antibody (mAb) was produced. Based on this mAb, a sensitive immunochromatographic strip assay (ICA) was established for rapid screening of propiconazole in vegetable samples. After optimisation of analytical parameters, the ICA strip showed a detection limit of 0.13 ng g −1 and a linear range from 0.5 to 80 ng g −1 using a strip reader. The assay also can be read by the naked eye with a visual limit of detection of 80 ng g −1 . The recoveries for spiked vegetable samples by ICA ranged from 85.2% to 114.9%, with a coefficient of variation less than 11.7%. The assay time is within 45 min for a single sample including the sample pre-treatment. For spiked and blind samples, the detection capability of ICA was equivalent to liquid chromatography-mass spectrometry. GRAPHICAL ABSTRACT: uf0001