Antioxidant properties of Klunzinger's mullet (Liza klunzingeri) protein hydrolysates prepared with enzymatic hydrolysis using a commercial protease and microbial hydrolysis with Bacillus licheniformis. (April 2022)

• Record Type: Journal Article
• Authors: Rabiei, Sana; Rezaei, Masoud; Nikoo, Mehdi; Khezri, Mohammad; Rafieian-Kopai, Mahmoud; Anjomshoaa, Maryam
• Is Part Of: Food science and technology international. Volume 28:Number 3(2022)
• Page Start: 233
• Page End: 246
• Publication Date: 2022-04
• Subjects: Bacillus licheniformis -- fermentation -- muscle hydrolysates -- hepatoprotective effects -- Liza klunzingeri; Food -- Analysis -- Periodicals; 664
• DOI: 10.1177/10820132211005297
• Languages: English
• ISSNs: 1082-0132

Abstract

Antioxidant activity of Klunzinger's mullet ( Liza klunzingeri ) muscle hydrolysates obtained using Bacillus licheniformis fermentation and enzymatic hydrolysis was determined. Hydrolysates obtained after 6 days of fermentation with B. licheniformis showed the highest free radical scavenging activity, metal chelating ability and ferric reducing antioxidant power (FRAP) (P ≤ 0.05). Microbial fermentation led to a higher percentage of small peptides and higher solubility compared with Alcalase hydrolysis (P ≤ 0.05). Hydrolysates showing the highest antioxidant properties attenuated serum, liver, and kidney oxidative stress biomarkers in male Wister rats stressed by carbon tetrachloride (P ≤ 0.05). At 300 mg/kg oral administration, hydrolysates increased serum, renal, and hepatic total antioxidant capacity (TAC) (P ≤ 0.05) and reduced their elevated levels of malondialdehyde (MDA), nitric oxide (NO ), and serum liver enzymes (AST, ALP, and ALT) (P ≤ 0.05). The hydrolysates were able to ameliorate hepatic damage by reducing necrosis, fatty changes, and inflammation. Results showed the antioxidant and hepato-toxic protective activities of Klunzinger's mullet muscle hydrolysates obtained using microbial fermentation, which may, therefore, potentially be considered as a functional food ingredient.