Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens. (October 2020)

• Record Type: Journal Article
• Authors: Ye, Xianfeng; Chen, Yang; Ma, Shiyun; Yuan, Tian; Wu, Yaxuan; Li, Yingxuan; Zhao, Yuqiang; Chen, Shuying; Zhang, Yiwen; Li, Liuyan; Li, Zhoukun; Huang, Yan; Cao, Hui; Cui, Zhongli
• Is Part Of: Food microbiology. Volume 91(2020)
• Publication Date: 2020-10
• Subjects: Volatile compounds -- Fusarium oxysporum f. sp. cucumerinum -- Penicillum digitatum -- Cell wall integrity -- Apoptosis-like death -- Postharvest storage; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2020.103502
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Myxobacteria have excellent biocontrol activity against various phytopathogens due to their rich spectrum of secondary metabolites and active predatory characteristics. In this study, the mycelial growth of Fusarium oxysporum f. sp. cucumerinum (FOC) was found to be significantly inhibited by volatile compounds (VOCs) produced by Corallococcus sp. EGB. A total of 32 compounds were identified among the VOCs produced by strain EGB, of which isooctanol exhibited the highest antifungal activity, with dosages of 3.75 and 4.0 μL/plate being sufficient to suppress FOC and Penicillum digitatum, respectively. Isooctanol was found to damage the cell wall and cell membranes of FOC and P. digitatum . Apoptosis-like cell death of FOC and P. digitatum induced by isooctanol was observed subsequently due to the accumulation of reactive oxygen species (ROS). The transcription level of genes related to cell wall integrity (CWI) pathway and redox reactions were significantly upregulated by 15- to 40-fold, indicating the stress caused by isooctanol. Postharvest storage experiments showed that the disease severity of post-harvest oranges infected with P. digitatum could be significantly reduced by isooctanol at 114.2 μL/L. Graphical abstract: Image 1 Highlights: Isooctanol exhibited the highest antifungal activity. Reactive oxygen species in isooctanol treated cells was accumulated significantly. Apoptosis-like cell death of FOC was induced by isooctanol treatment. The disease severity of oranges infected with P. digitatum could be significantly reduced by isooctanol.