Click reaction triggered turn-on fluorescence strategy for highly sensitive and selective determination of steroid hormones in food samples. (16th April 2022)

• Record Type: Journal Article
• Authors: Li, Qianyu; Li, Guoliang; Fan, Lihua; Yu, Yanxin; Liu, Jin
• Is Part Of: Food chemistry. Volume 374(2022)
• Publication Date: 2022-04-16
• Subjects: Steroid hormones -- CuAAC -- Fluorescence derivatization -- Food samples; CAN acetonitrile -- CuAAC copper(I)-catalyzed azide-alkyne cycloaddition -- CuSO4 sulfate pentahydrate -- DMF N, N-dimethylformamide -- EDCs endocrine disruptors -- EE2 ethinylestradiol -- EFSA European Food Safety Authority -- EtOH ethanol -- G gestodene -- GC–MS gas chromatography with mass spectrometry -- HPLC-FLD high performance liquid chromatography with fluorescence detection -- L-AA l-Ascorbic acid -- LC-MS liquid chromatography tandem mass spectrometry -- LOD limit of detection -- LOQ limit of quantification -- MeOH methanol -- NA norethisteron acetate -- NGT norgestrel -- THF tetrahydrofuran -- 19-NTD norethindrone; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131565
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A "turn-on" fluorescence click labeling method was used to determine steroid hormones. The 3-Azido-7-hydroxycoumarin was first used as a derivative reagent. The method is of superior selectivity for detection of steroid hormones in food samples. Abstract: The copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction has becoming noticeable in the field of analytical chemistry. Mild reaction conditions, simple operation, high efficiency, and good regioselectivity make this classical click reaction a perfect strategy for chemical derivatization. Herein, we proposed a promising click fluorescent labeling method with high selectivity for the determination of five steroid hormones in food samples. The labeling strategy depends on the reaction between 3-Azido-7-hydroxycoumarin and the alkynyl group of steroid hormones, which shows a turn-on fluorescence response in the presence of copper (I). The formed fluorescent products were detected by HPLC-FLD. Under the optimized conditions, the proposed method presented excellent performance with good linearity (R 2 ≥ 0.9998) and low detection limit (1.8–7.3 μg L -1 ). Further, satisfactory recoveries were obtained to be 82–107% in spiked meats with relative standard deviations (RSDs) ≤ 5.7%. Finally, the established method was successfully applied for the determination of steroid hormones in meat, indicating the potential prospect of the click reaction in chemical derivatization.