Multi-residue method validation and safety evaluation of pesticide residues in seed spices cumin (Cuminum cyminum) and coriander (Coriandrum sativum) by gas chromatography tandem mass spectrometry (GC–MS/MS). (16th April 2022)

• Record Type: Journal Article
• Authors: Girame, Rushali; Shabeer, T.P. Ahammed; Ghosh, Bappa; Hingmire, Sandip; Natarajan, Ramesh; Dubey, Pradyumn Narayan
• Is Part Of: Food chemistry. Volume 374(2022)
• Publication Date: 2022-04-16
• Subjects: Pesticide residues -- Multi-residue validation -- Safety evaluation -- Cumin and coriander -- GC–MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131782
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A robust analytical method for 215 pesticide residues is established in cumin and coriander by GC–MS/MS analysis. Sample preparation involved QuEChERS extraction, d-SPE cleanup followed by reconstituted in ethyl acetate. The method complies with SANTE/12682/2019 guideline for method validation. This method has potential application for regulatory analysis of pesticide residue in cumin and coriander. Abstract: The manuscript reports comprehensive multi-residue determination of 215 pesticides in two commercially important Indian spices, Cumin ( Cuminum cyminum) and Coriander (Coriandrum sativum) by GC–MS/MS analysis. The proposed method involved liquid–liquid extraction with acetonitrile, d-SPE clean-up and final reconstitution of extract in ethyl acetate. d-SPE clean-up with PSA and C18 minimized the matrix effects by 40 and 16%, respectively. Reconstitution of final extract reduced the non-volatile matrix co-extractives by 36–40%. The method was validated as per SANTE/12682/2019 and recoveries at 10, 25 and 50 μg kg −1 were within 70–120% with RSD ≤ 20%. A fit for purpose method LOQ of 10 μgkg −1 was achieved for 85% of analytes. The method was successfully applied for comprehensive screening of cumin and coriander market samples. The calculated TMDI for acute and chronic exposure assessment were less than calculated MPI in respective matrices and therefore did not cause any adverse effect to consumers.