Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine. Issue 17 (12th April 2019)

• Record Type: Journal Article
• Authors: Guo, Jianshuang; Zhang, Guoqiang; Ma, Jing; Zhao, Caili; Xue, Qingqing; Wang, Jiyan; Liu, Wenjie; Liu, Kaihui; Wang, Haifeng; Liu, Ning; Song, Qitao; Li, Jing
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 17(2019)
• Page Start: 4326
• Page End: 4334
• Publication Date: 2019-04-12
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c9ob00516a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Ac3 6AzGalNAc, as a metabolic probe, can robustly label O -GlcNAc-modified proteins in cells with high selectivity. Abstract : An unnatural monosaccharide with a C6-azide, Ac3 6AzGalNAc, has been developed as a potent and selective probe for O -GlcNAc-modified proteins. Combined with click chemistry, we demonstrate that Ac3 6AzGalNAc can robustly label O -GlcNAc glycosylation in a wide range of cell lines. Meanwhile, cell imaging and LC-MS/MS proteomics verify its selective activity on O -GlcNAc. More importantly, the protocol presented here provides a general methodology for tracking, capturing and identifying unnatural monosaccharide modified proteins in cells or cell lysates.