Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine. Issue 17 (12th April 2019) - Guo, Jianshuang
Abstract : Ac3 6AzGalNAc, as a metabolic probe, can robustly label O -GlcNAc-modified proteins in cells with high selectivity. Abstract : An unnatural monosaccharide with a C6-azide, Ac3 6AzGalNAc, has been developed as a potent and selective probe for O -GlcNAc-modified proteins. Combined with click chemistry, we demonstrate that Ac3 6AzGalNAc can robustly label O -GlcNAc glycosylation in a wide range of cell lines. Meanwhile, cell imaging and LC-MS/MS proteomics verify its selective activity on O -GlcNAc. More importantly, the protocol presented here provides a general methodology for tracking, capturing and identifying unnatural monosaccharide modified proteins in cells or cell lysates.