Development of an indirect competitive enzyme-linked immunosorbent assay for propiconazole based on monoclonal antibody. (April 2022)

• Record Type: Journal Article
• Authors: Li, Jiao; Ding, Yuan; Chen, He; Sun, Wanlin; Huang, Yue; Liu, Fengquan; Wang, Minghua; Hua, Xiude
• Is Part Of: Food control. Volume 134(2022)
• Publication Date: 2022-04
• Subjects: Propiconazole -- Hapten -- Monoclonal antibodies -- Indirect competitive enzyme-linked immunosorbent assay -- Pesticide residues; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2021.108751
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Propiconazole is a widely used fungicide in agricultural production, but it poses risks to human health. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) was established to detect propiconazole residues. Three haptens of propiconazole were designed and synthesized to prepare antigens. Ten mAbs were obtained using hapten B (5-(4-(2-((1H-1, 2, 4-triazol-1-yl)methyl)-4-propyl-1, 3-dioxolan-2-yl)-3-chlorophenyl) pentanoic acid). The ic-ELISA based on the combination of mAb 2G2E12 and hapten B-ovalbumin (coating antigen) showed the highest sensitivity. After optimization, the 50% inhibition concentration, limit of detection and linear range of the ic-ELISA were 2.33 μg/L, 0.26 μg/L and 0.26–21.28 μg/L, respectively. The cross-reactivities for the analogs of propiconazole were ≤1.9%. The average recoveries of the spiked samples were 75.7%–117.0% with relative standard deviations of ≤9.4%. The results of the ic-ELISA were consistent with those of gas chromatography-mass spectrometry in the analysis of blind samples. Therefore, we provide an alternative tool for the accurate and reliable determination of propiconazole. Highlights: Three haptens of propiconazole was designed and synthesized successfully. Ten mAbs were prepared and the optimal combination of mAb and antigen was obtained. An ic-ELISA for propiconazole with high sensitivity and specificity was developed. The ic-ELISA was successfully used to detect propiconazole in agro-products. The ic-ELISA had good correlation with GC-MS in the detection of propiconazole.