Bio-affinity ultra-filtration combined with HPLC-ESI-qTOF-MS/MS for screening potential α-glucosidase inhibitors from Cerasus humilis (Bge.) Sok. leaf-tea and in silico analysis. (30th March 2022)

• Record Type: Journal Article
• Authors: Li, Songjie; Wang, Ruimin; Hu, Xiaoping; Li, Congfa; Wang, Lu
• Is Part Of: Food chemistry. Volume 373:Part B(2022)
• Publication Date: 2022-03-30
• Subjects: CLT Cerasus humilis (Bge.) Sok. leaf-tea -- TPC total phenolic content -- TFC total flavonoid content -- α-GIA α-glucosidase inhibitory activity -- α-GIs α-glucosidase inhibitors -- MeOH methanol -- EtOH ethanol -- EtAc ethyl acetate -- DW dried weight -- RT retention time -- DMSO dimethyl sulfoxide -- BD bio-affinity degree -- HPLC-ESI-qTOF/MS high-performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry; Cerasus humilis (Bge.) Sok -- Phenolic composition -- Bio-affinity ultrafiltration -- HPLC-ESI-qTOF-MS/MS -- α-glucosidase inhibitors -- In silico analysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131528
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Cerasus humilis leaf-tea (CLT) was a good source rich in α -glucosidase inhibitors ( α -GIs) Phenolic compositions of the CLT extracts were identified for the first time. Ultra-filtration plus LC-MS/MS technique was used to screen the α -GIs rapidly. Myricetin, avicularin, quercitrin, prunin and guajavarin were major α -GIs in CLT. Interactions mechanisms of the screened α -GIs on α -glucosidase were clarified. Abstract: Cerasus humilis (Bge.) Sok. leaf-tea (CLT) has a potential anti- α -glucosidase effect. However, its anti- α -glucosidase functional compositions remain unclear. Results showed that 70% methanol extract of CLT (IC50 = 36.57 μg/mL) with the highest total phenolic/flavonoid contents exhibited significantly higher α -glucosidase inhibitory activity ( α -GIA) than acarbose (IC50 = 189.57 μg/mL). Additionally, phenolic constituents of the CLT extract were analyzed for the first time in this work. Ten major potential α -glucosidase inhibitors ( α -GIs) with high bio-affinity degree in the CLT extract were recognized using a bio-affinity ultra-filtration and HPLC-ESI-qTOF-MS/MS method. In vitro α -GIA assay confirmed that myricetin (IC50 = 36.17 μg/mL), avicularin (IC50 = 69.84 μg/mL), quercitrin, isoquercitrin, prunin and guajavarin were responsible for the α -GIA of the CLT extract. More importantly, the interaction mechanism between α -GIs and α -glucosidase was investigated via in silico analysis. This study provides a high-throughput screening platform for identification of the potential α -GIs from natural products.