Method development and validation for analysis of phenolic compounds in fatty complex matrices using enhanced matrix removal (EMR) lipid cleanup and UHPLC-QqQ-MS/MS. (30th March 2022)

• Record Type: Journal Article
• Authors: Yin, Zhiya; Yuan, Bo; Lyu, Weiting; Huang, Qingrong; Simon, James E.; Wu, Qingli
• Is Part Of: Food chemistry. Volume 373:Part A(2022)
• Publication Date: 2022-03-30
• Subjects: Phenolic compounds -- High-fat matrices -- Enhanced matrix removal -- Solid phase extraction -- QuEChERS -- UHPLC-QqQ-MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.131096
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A robust UHPLC-QqQ-MS/MS method developed for 55 phenolic compounds in 7 fatty matrices. QuEChERS and enhanced matrix removal (EMR) in 96-well plate for sample cleanup. EMR removed 56–77% of total lipids as profiled by UHPLC-QTOF-MS. Stepwise recoveries were validated, with final accuracy of 78–117%. Abstract: Reliable analysis of phenolic compounds in fatty matrices is a challenging task. In this work, a robust analytical method was developed and validated for 55 phenolic compounds employing QuEChERS (quick, efficient, cheap, easy, rugged and safe) and Enhanced Matrix Removal (EMR)-lipid cleanup in 96-well plates for sample preparation, coupled with ultra-high performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). Seven high-fat matrices of pork brain, belly and liver; horse serum, beef, salmon and avocado were explored for method validation and led to promising stepwise recoveries of extraction, clean-up, drying-reconstitution of most analytes ranging from 75% to 113%, and with an accuracy of 78%∼117%, except for six catechin-analogues. The matrix removal efficiency of EMR was determined using UHPLC-quadruple time of flight (QTOF)-MS, and results indicated that 56%∼77% of co-extractives were removed. This method would be readily extended to wide range of applications demanding high-throughput and sensitive analysis of phenolic compounds in fatty samples.