Synthesis and characterization of cellobiose-derived oligosaccharides with Bifidogenic activity by glucansucrase E81. (December 2021)

• Record Type: Journal Article
• Authors: İspirli, Hümeyra; Bowman, Michael J.; Skory, Christopher D.; Dertli, Enes
• Is Part Of: Food bioscience. Volume 44:Part A(2021)
• Publication Date: 2021-12
• Subjects: Glucansucrase -- Prebiotic oligosaccharides -- Cellobiose-derived oligosaccharides; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2021.101388
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Cellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an α-linked glucose to cellobiose at either the 2- O position of the reducing end (86%) glucose or the 6- O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and α-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.