Lactobacillus casei LC89 exerts antidiabetic effects through regulating hepatic glucagon response and gut microbiota in type 2 diabetic mice. Issue 18 (26th July 2021)

• Record Type: Journal Article
• Authors: Zhang, Yongli; Wu, Tao; Li, Wen; Zhao, Yunjiao; Long, Hairong; Liu, Rui; Sui, Wenjie; Zhang, Min
• Is Part Of: Food & function. Volume 12:Issue 18(2021)
• Page Start: 8288
• Page End: 8299
• Publication Date: 2021-07-26
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo00882j
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Lactobacillus casei LC89 exhibits remarkable anti-diabetic effects by inhibiting hepatic glucagon action and reshaping the gut microbiota, and might be considered as a dietary supplement to regulate glucose metabolism. Abstract : Previous study suggests that Lactobacillus casei exhibits antihyperglycemic activity, however, the molecular mechanism of this has yet to be elucidated. Here, the anti-diabetic effects and underlying mechanisms of Lactobacillus casei LC89 are investigated in type 2 diabetes mellitus (T2DM) mice, which was induced by a high-fat diet (HFD) with streptozotocin (100 mg per kg BW). The results show that LC89 at a dose of 10 9 CFU day −1 decreases fasting blood glucose (FBG) and insulin levels by 35.12% and 28.37%, respectively, compared to the diabetes control (DC) group. Moreover, LC89 treatment improved the insulin resistance index (HOMA-IR), serum lipid profiles and inflammation cytokines. The real-time polymerase chain reaction indicated that LC89 markedly downregulates the mRNA expression of hepatic glucagon (GCG), glucagon receptor (GCGR), phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). Meanwhile, LC89 significantly decreases the abundance of Odoribacter, but increases the Alloprevotella, Bacteroides, Parabacteroides and Ruminococcus content. Therefore, LC89 plays a positive role in alleviating T2DM by regulating gut microbiota and glucagon signal pathway-related genes, and it may be a beneficial dietary supplement to regulate glucose metabolism in T2DM.