Bioactivity screening of pinhão (Araucaria Angustifolia (Bertol.) Kuntze) seed extracts: the inhibition of cholinesterases and α-amylases, and cytotoxic and anti-inflammatory activities. Issue 20 (26th August 2021)

• Record Type: Journal Article
• Authors: de Oliveira, Anielle; Moreira, Thaysa Fernandes Moya; Pepinelli, Ana Luisa Silva; Costa, Luis Gustavo Médice Arabel; Leal, Luana Eloísa; da Silva, Tamires Barlati Vieira; Gonçalves, Odinei Hess; Porto Ineu, Rafael; Dias, Maria Inês; Barros, Lillian; Abreu, Rui M. V.; Ferreira, Isabel C. F. R.; Bracht, Lívia; Leimann, Fernanda Vitória
• Is Part Of: Food & function. Volume 12:Issue 20(2021)
• Page Start: 9820
• Page End: 9828
• Publication Date: 2021-08-26
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo01163d
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : The potential bioactive properties of extracts from bio-residues of pinhão ( Araucaria angustifolia (Bertol.) Kuntze) seeds were evaluated, namely the α-amylase and cholinesterase inhibition, cytotoxicity, and anti-inflammatory properties. Abstract : The objective of this work was to determine the potential bioactive properties of extracts from bio-residues of pinhão ( Araucaria angustifolia (Bertol.) Kuntze) seeds, namely the α-amylase and cholinesterase inhibition, cytotoxicity, and anti-inflammatory properties. The pinhão extracts evaluated were obtained from cooking water (CW) and as an ethanolic extract from residual pinhão seed shells (PS). Catechin was the major compound found in both extracts. The PS extract presented higher antioxidant levels and the better inhibition of human salivary and porcine pancreatic α-amylases when compared to the CW extract. Also, based on in vivo evaluations, the PS extract did not differ significantly from acarbose when compared to a control group. The most potent inhibitor of cholinesterases was the CW extract. No cytotoxicity toward normal cells was detected, and neither extract showed anti-inflammatory activity. The PS extract presented cytotoxic activity toward non-small-cell lung, cervical, hepatocellular and breast carcinoma cell lines. Overall, the results demonstrated the potential bioactivity of extracts obtained from pinhão bio-residues.