Nanoparticles composed of the tea polysaccharide-complexed cationic vitamin B12-conjugated glycogen derivative. Issue 18 (27th July 2021)

• Record Type: Journal Article
• Authors: Mao, Xuhong; Long, Lingli; Shen, Juncheng; Lin, Kunhua; Yin, Lin; Yi, Juzhen; Zhang, Li-Ming; Deng, David Y. B.; Yang, Liqun
• Is Part Of: Food & function. Volume 12:Issue 18(2021)
• Page Start: 8522
• Page End: 8534
• Publication Date: 2021-07-27
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo00487e
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine. Abstract : Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine. To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12 -conjugated glycogen derivative bearing the diethylenetriamine residues (VB12 -DETA-Gly) was synthesized and characterized using FTIR, 1 H NMR, and UV-vis spectroscopy. An acidic tea polysaccharide (TPSA) was isolated from green tea. The TPSA/VB12 -DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50–100 nm. To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized. The interactions and complexation mechanism between the CR-TPSA and the VB12 -DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy. The results indicated that the electrostatic interaction could play a major role during the CR-TPSA and VB12 -DETA-Gly-II complexation processes. The TPSA/VB12 -DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model. Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.