Salmonella enterica subsp. enterica virulence potential can be linked to higher survival within a dynamic in vitro human gastrointestinal model. (February 2022)

• Record Type: Journal Article
• Authors: Cavestri, Camille; Savard, Patricia; Fliss, Ismail; Emond-Rhéault, Jean-Guillaume; Hamel, Jérémie; Kukavica-Ibrulj, Irena; Boyle, Brian; Daigle, France; Malo, Danielle; Bekal, Sadjia; Harris, Linda J.; Levesque, Roger C.; Goodridge, Lawrence; LaPointe, Gisèle
• Is Part Of: Food microbiology. Volume 101(2022)
• Publication Date: 2022-02
• Subjects: Salmonella enterica -- Gastrointestinal model -- Virulence -- Survival variability; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2021.103877
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Salmonella enterica subsp. enterica is one of the leading causes of human foodborne infections and several outbreaks are now associated with the consumption of fresh fruit and vegetables. This study aims at evaluating whether Salmonella virulence can be linked to an enhanced ability to survive successive digestive environments. Thirteen S. enterica strains were selected according to high and low virulence phenotypes. Lettuce inoculated separately with each S. enterica strain was used as food matrix in the TNO gastrointestinal model (TIM-1) of the human upper gastrointestinal tract. During the passage in the stomach, counts determined using PMA-qPCR were 2–5 logs higher than the cultivable counts for all strains indicating the presence of viable but non-cultivable cells. Bacterial growth was observed in the duodenum compartment after 180 min for all but one strain and growth continued into the ileal compartment. After passage through the simulated gastrointestinal tract, both virulent and avirulent S. enterica strains survived but high virulence strains had a significantly ( p = 0.004) better average survival rate (1003 %–3753 %) than low virulence strains (from 25 % to 3730%). The survival rates of S. enterica strains could be linked to the presence of genes associated with acid and bile resistance and their predicted products. The presence of single nucleotide polymorphisms may also impact the function of virulence associated genes and play a role in the resulting phenotype. These data provide an understanding of the relationship between measured virulence potential and survival of S. enterica during dynamic simulated gastrointestinal transit. Highlights: PMA-qPCR revealed viable but non cultivable Salmonella cells during digestion. All but one Salmonella strain was able to grow under duodenum and ileal conditions. Average survival rate was better for high virulence strains than for low virulence strains.