Hydrolyzed collagen from defatted sea bass skin and its conjugate with epigallocatechin gallate: In vitro antioxidant, anti-inflammatory, wound-healing and anti-obesity activities. (October 2021)

• Record Type: Journal Article
• Authors: Chotphruethipong, Lalita; Binlateh, Thunwa; Hutamekalin, Pilaiwanwadee; Sukketsiri, Wanida; Aluko, Rotimi E.; Benjakul, Soottawat
• Is Part Of: Food bioscience. Volume 43(2021)
• Publication Date: 2021-10
• Subjects: Sea bass skin -- Hydrolyzed collagen -- Conjugated peptides -- Epigallocatechin gallate -- Wound-healing -- Anti-inflammation -- Anti-obesity; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2021.101303
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The in vitro antioxidant, anti-inflammatory, wound-healing and anti-obesity activities of hydrolyzed collagen (HC) from defatted sea bass ( Lates calcarifer ) skin and HC conjugated with epigallocatechin gallate (EGCG) were comparatively studied. HC and HC-EGCG conjugate at all levels (1.25–10 mg/mL) had H2 O2 scavenging activity, but the conjugate was more active at all levels ( P < 0.05). Both samples (50–200 μg/mL) exhibited anti-inflammatory activity by inhibiting nitric oxide and tumor necrosis factor-α production in lipopolysaccharide-induced RAW264.7 cells but HC at 200 μg/mL exhibited the highest inhibitory activities ( P < 0.05). When HaCaT keratinocyte cells were treated with both samples, no significant ( P < 0.05) cytotoxicity was found at all levels (50–200 μg/mL). Moreover, HC at 200 μg/mL stimulated the highest level of cell migration ( P < 0.05), which reflects a better wound healing effect. The HC-EGCG conjugate at 5.0–6.25 mg/mL showed stronger inhibitions of α-amylase and α-glucosidase activities and had potentially better anti-diabetic effect than HC ( P < 0.05). Graphical abstract: Image 1 Highlights: Sea bass skin hydrolyzed collagen and its conjugate had H2 O2 scavenging activity. Both samples showed activity towards pro-inflammatory mediators of macrophage cell. Both samples could also promote migration and filipodia formation of HaCaT cell. Activities of obesity-related enzymes were inhibited by both samples.