Folium nelumbinis (Lotus leaf) volatile-rich fraction and its mechanisms of action against melanogenesis in B16 cells. (15th November 2020)

• Record Type: Journal Article
• Authors: Teng, Hui; Fan, Xiaoyun; Lv, Qiyan; Zhang, Qin; Xiao, Jianbo; Qian, Yuewei; Zheng, Baodong; Gao, Hui; Gao, Sihai; Chen, Lei
• Is Part Of: Food chemistry. Volume 330(2020)
• Publication Date: 2020-11-15
• Subjects: F. nelumbinis -- Petroleum ether fraction -- Volatile compound -- Tyrosinase inhibition -- B16 cell -- Apoptosis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127030
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Lotus leaf oil could effectively inhibit melanin production. Lotus leaf oil might be used as a potent tyrosinase inhibitor against melanoma. Lotus leaf oil induces in B16 cells apoptosis. Abstract: This study was aimed at determining the influence of Folium nelumbinis (Lotus leaf) extracts on melanogenesis in vitro models of melanoma cell line. The anticancer activity of four fractions, including petroleum ether (PEE), n -hexane (HE), ethanol (EE), and ethyl acetate (EAE) from F. nelumbinis on B16 cell lines (C57BL/6J melanoma cell), were evaluated after 24 and 48 h treatment. Results showed that PEE as well as volatile-rich fractions of linolenic acid and linolenic acid ethyl ester significantly (p < 0.05) reduced tyrosinase activity and melanin content in B16 melanoma cells model. Meanwhile, PEE and its primarily contained compound triggered apoptosis of B16 cells in a dose-dependent way. These results demonstrated that PEE possessed effective activities against melanin and tyrosinase generations through the induction of apoptosis. Moreover, a relation between the volatile-rich fractions of F. nelumbinis and the anticancer effects was demonstrated as well.