Anti-degranulation and bile acid-binding activity of extracts from fruits and agro-industrial by-products. (October 2021)

• Record Type: Journal Article
• Authors: Hamauzu, Yasunori; Kawahara, Takeshi
• Is Part Of: Food bioscience. Volume 43(2021)
• Publication Date: 2021-10
• Subjects: Fruit by-products -- Agro-industrial wastes -- Proanthocyanidins -- Anti-degranulation -- Bile acid-binding; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2021.101244
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: To evaluate the utility of fruits and by-products/wastes as bioactive materials, we investigated the anti-allergic effects of hot-water and ethanol extracts from various fruit by-products and medicinal fruits using a β-hexosaminidase release assay. Bile acid-binding ability, which is related to the acceleration of bile acid excretion, was also determined for hot-water extracts. Among the extracts, the ethanol extract of grape bunch stem showed the highest (63.6%) inhibition of degranulation, as assessed by β-hexosaminidase release from rat basophilic leukemia cells (RBL-2H3) at a dose of 100 μg/mL. The hot-water extract from grape seed contained the highest total polyphenol content (353 mg gallic acid equivalent/g dry extract), but the total polyphenol content did not show a high correlation with the anti-degranulation effect. A positive relationship between the inhibitory effect of degranulation and proanthocyanidin content (0.1–0.3 in A 550 ) was observed, but higher concentrations of proanthocyanidins in the system showed adverse effects. Proanthocyanidin content was also related to the bile acid-binding ability of the hot-water extracts with the highest value from Chinese quince hot-water extract (95%, 47.7 mg/g dry extract). These results suggest that proanthocyanidin-rich extracts from fruits and agro-industrial by-products have bioactive potential and can be exploited as anti-degranulation and bile acid-eliminating materials. Highlights: Extracts of fruits and agro-industrial by-products were analyzed. Grape bunch stem ethanol extract showed the highest anti-degranulation effect. Chinese quince hot-water extract showed the highest bile acid-binding ability. Proanthocyanidins were important for anti-degranulation and bile acid-binding activities. High doses of proanthocyanidins had negative effects on anti-degranulation.