Selective extraction and determination of steroidal glycoalkaloids in potato tissues by electromembrane extraction combined with LC-MS/MS. (15th January 2022)

• Record Type: Journal Article
• Authors: Wan, Libin; Gao, Haidong; Gao, Huoliang; Du, Rui; Wang, Fayun; Wang, Yong; Chen, Mantang
• Is Part Of: Food chemistry. Volume 367(2022)
• Publication Date: 2022-01-15
• Subjects: Electromembrane extraction -- Steroidal glycoalkaloids -- Bioactive metabolites -- Potato -- Plant tissue; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130724
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: EME was first used to extract steroidal glycoalkaloids in complex matrices. EME showed high extraction efficiency and sufficient sample clean-up ability. EME-LC-MS/MS was successfully used to analyze steroidal glycoalkaloids in potatoes. Wide linear correlation, good accuracy and low detection limits were confirmed. Abstract: For the first time, electromembrane extraction (EME) combined LC-MS/MS was applied to extract and determine α-solanine and α-chaconine in different potato tissues using NPOE containing 20% (v/v) DEHP as supported liquid membrane (SLM). Under the optimal conditions, the proposed EME-LC-MS/MS method was evaluated using spiked fresh potato peel sample. The linear range for α-solanine and α-chaconine was 5–1000 ng mL −1 (R 2 > 0.9991), with LOD and LOQ of 1.2–1.5 ng mL −1 and 4.1–5.2 ng mL −1, respectively. Repeatability for α-solanine and α-chaconine at three concentration levels was satisfactory (<4.9%), and recoveries ranged from 73% to 106%. Finally, the EME-LC-MS/MS method has been successfully employed to determine α-solanine and α-chaconine in sprouted potato peel and tuber samples, indicating that EME exhibited high selectivity and efficient sample clean-up capability. Consequently, EME showed great potential for extraction and purification of toxic and bioactive basic compounds from complex plant tissues.