Controllable assembly metal-organic frameworks and gold nanoparticles composites for sensitive immunochromatographic assay. (15th January 2022)

• Record Type: Journal Article
• Authors: Yin, Xuechi; Dou, Leina; Yao, Xiaolin; Liu, Sijie; Zhang, Liang; Zhao, Man; Su, Lihong; Sun, Jing; Wang, Jianlong; Zhang, Daohong
• Is Part Of: Food chemistry. Volume 367(2022)
• Publication Date: 2022-01-15
• Subjects: Immunochromatographic assay -- Metal-organic frameworks -- AuNPs -- Label strategy -- Furazolidone; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130737
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: The NU66@AuNPs surmount MOFs' defects in optical property and biocompatibility. The NU66@AuNPs has a superiority in resisting interference from salt ions. NU66 used here possesses more stronger load capacity and controllable properties. The NU66@AuNPs-ICA has excellent detection performance and specificity for CPAOZ. Abstract: Creating universal signal labels from fundamental building blocks with excellent biocompatibility and well-controlled size/uniformity simultaneously for immunochromatographic assay (ICA) is highly desired but extremely challenging. Here, a nano-signal label strategy was reported, in which the amino-terminated zirconium MOFs (NU66) are adopted to construct powerful matrix materials and gold nanoparticles (AuNPs) act as the linker between metal-organic frameworks (MOFs) and antibodies. Particularly, AuNPs were immobilized directly on the surface of NU66, giving NU66 excellent biocompatibility with bright color signal labels and improving the salt ion stability of AuNPs. As a proof of concept, the furazolidone residues was monitored by the developed NU66@AuNPs-ICA in food samples (pork, shrimp and eggs). With 3-[(4-carboxyphenyl) monomethyl] amino-2-oxazolidinone (CPAOZ) as analyte target, the visual limit of detection (vLOD) and cut-off level were 0.6 ng/mL and 3.0 ng/mL, respectively. This work may open a new avenue for the application of MOFs in immunochromatography assays.