Bacteriophages LSA2308 and LSA2366 infecting drug-resistant Staphylococcus aureus: Isolation, characterization and potential application for milk safety. (December 2021)

• Record Type: Journal Article
• Authors: Ma, Fei; Ning, Yu; Wan, Qingqing; Zou, Likou; Liu, Yuntao; Chen, Shujuan; Li, Jianlong; Zeng, Zhen; Yang, Yong; Chen, Hong; Zhou, Min; Liu, Shuliang; Li, Cheng; Liu, Aiping
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 152(2021)
• Publication Date: 2021-12
• Subjects: Bacteriophage -- Biocontrol -- Cocktail -- Drug resistance -- Staphylococcus aureus; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2021.112298
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Bacteriophages are considered a promising candidate for controlling food pathogens. In this study, we isolated and characterized S . aureus- infecting bacteriophages, and investigated their potential for antibiotic substitute and bacteriophage cocktail effect in Luria-Bertani (LB) broth and skim milk. Bacteriophages LSA2308 and LSA2366 possessing lytic activity against drug-resistant S. aureus were isolated. The latent period of LSA2308 and LSA2366 was approximately 20 and 10 min, with burst size of 407 and 258 PFU per infected cell, respectively. A combination of LSA2308 (or LSA2366) and 1/2 MIC ampicillin achieved better clearance of S. aureus CR003 in LB broth than treatment with either bacteriophage or ampicillin alone. Biocontrol with 10 5 –10 7 PFU/mL LSA2308 or LSA2308/LSA2366 cocktail was effective in LB broth, whereas it was less active in skim milk. Compared with treatment using 10 5 or 10 6 PFU/mL bacteriophages, the bactericidal efficacy of 10 7 PFU/mL bacteriophages on single or mixed S. aureus strains in skim milk was the best at 4 and 25 °C, revealing a dose-dependent mode. Our study further expands the repertoire of bacteriophages infecting drug-resistant S. aureus, and provides insights into the control of S. aureus in milk production chain. Highlights: Phages LSA2308 and LSA2366 with lytic activity against drug-resistant foodborne S. aureus were isolated. Combination of phage and ampicillin achieved better biocontrol than treatment with either phage or ampicillin alone. Phages LSA2308 and LSA2366 showed application potentials in milk production chain.