Collagen peptides with DPP-IV inhibitory activity from sheep skin and their stability to in vitro gastrointestinal digestion. (August 2021)

• Record Type: Journal Article
• Authors: Wang, Beibei; Yu, Zhe; Yokoyama, Wallace; Chiou, Bor-Sen; Chen, Maoshen; Liu, Fei; Zhong, Fang
• Is Part Of: Food bioscience. Volume 42(2021)
• Publication Date: 2021-08
• Subjects: Collagen peptides -- Sheep skin -- Dipeptidyl peptidase IV inhibitory -- LC−MS/MS identification -- Gastrointestinal digestion stability; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2021.101161
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Collagen is a potential precursor protein for Dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides because of its high content of Pro. Collagen peptides with DPP-IV inhibitory activity were prepared and identified from sheep skin. The peptide sequences with the highest potential for DPP-IV inhibition were recognized by nano LS-MS/MS and in silico analysis. DPP-IV inhibitory activity of collagen peptides usually decreased after digestion, therefore the effect of peptides digestion on DPP-IV inhibitory activity were studied. Four DPP-IV inhibitory peptides resistant to digestive enzymes or having the possibility of generating new DPP-IV inhibitory peptides after digestion were synthesized. The activity of synthetic peptides before and after digestion were also studied. GPAGPIGPV was resistant to digestion and GPAGPOGFPG discharged DPP-IV inhibitory peptides in silico digestion, suggesting that they can maintain high DPP-IV inhibitory activity after digestion. Binding sites of the peptides with DPP-IV were studied by molecular docking, indicating the mechanism of the peptide having DPP-IV inhibitory activity. The results indicated that DPP-IV-inhibitory peptides identified from sheep skin collagen may be functional components in diabetic diet. Graphical abstract: Image 1 Highlights: Collagen peptides with DPP-IV inhibitory activity were obtained from sheepskin. ACP sample had highest oligopeptide content with best DPP-IV inhibitory activity. GPAGPIGPV and GPAGPOGFPG showed the highest DPP-IV-inhibitory activity GPAGPIGPV could be protected from digestion. GPAGPOGFPG could release other DPP-IV inhibitory peptides upon simulated digestion.