Dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay for simultaneous detection of aflatoxin B1 and zearalenone in feedstuffs. (1st January 2022)

• Record Type: Journal Article
• Authors: Li, Runxian; Wen, Yang; Yang, Luqing; Liu, Anguo; Wang, Fenglai; He, Pingli
• Is Part Of: Food chemistry. Volume 366(2022)
• Publication Date: 2022-01-01
• Subjects: Quantum dot nanobeads -- Mycotoxins -- Fluorescence-linked immunosorbent assay -- Simultaneous detection -- Feedstuffs; QBs Quantum dot nanobeads -- FLISA Fluorescence-linked immunosorbent assay -- AFB1 Aflatoxin B1 -- ZEN Zearalenone -- MRLs Maximum residue levels -- mAbs Monoclonal antibodies -- IgG Immunoglobulin -- FAPAS Food Analysis Performance Assessment Scheme -- PBS phosphate buffer saline -- TEM Transmission electron microscopy -- ZAN Zearalanone -- α-ZOL α-zearalenol -- β-ZOL β-zearalanol -- FB1 Fumonisin B1 -- LC-MS/MS Liquid chromatography tandem mass spectrometry -- OTA Ochratoxin A -- ELISA Enzyme-linked immunosorbent assay -- AFB2 Aflatoxin B2 -- AFG1 Aflatoxin G1 -- AFG2 Aflatoxin G2 -- DON Deoxynivalenol -- TMB 3, 3′, 5, 5′-tetramethyl benzidine -- EDC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide -- BSA Bovine serum albumin -- PB Phosphate buffer -- LOD Limit of detection -- RSD Relative standard deviation -- DLS Dynamic light scattering -- CR Cross reactivity -- PBST Phosphate buffer saline containing Tween20; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130527
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Dual quantum dot nanobeads were applied in fluorescence-linked immunosorbent assay (QBs-FLISA). Two target mycotoxins can be detected simultaneously by QBs-FLISA. Compared with ELISA, detection sensitivity of QBs-FLISA was increased and detection duration was decreased. QBs-FLISA was successfully applied in feedstuff analysis. Abstract: A novel dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay (QBs-FLISA) was successfully developed for simultaneously detecting aflatoxin B1 (AFB1) and zearalenone (ZEN) in feedstuffs. Dual CdSe/ZnS quantum dot nanobeads with different diameters that emit red and green fluorescence were conjugated with anti-AFB1 and anti-ZEN monoclonal antibodies to prepare fluorescent probes, which greatly enhance analytical performance. Under the optimal conditions, the limits of detection for AFB1 and ZEN were 9.3 and 102.1 pg mL −1, respectively. The recoveries ranged from 82.50% to 116.21% with relative standard deviation less than 11.3%. Compared with traditional enzyme-linked immunosorbent assay, detection sensitivities of AFB1 and ZEN using QBs-FLISA were increased 20 and 5 folds, respectively. In addition, results of feedstuff samples analyzed by QBs-FLISA and liquid chromatography tandem mass spectrometry showed a good agreement (R 2 = 0.99).