Development of a label free electrochemical sensor based on a sensitive monoclonal antibody for the detection of tiamulin. (1st January 2022)

• Record Type: Journal Article
• Authors: Wang, Aiping; You, Xiaojuan; Liu, Hongliang; Zhou, Jingming; Chen, Yumei; Zhang, Chenyang; Ma, Kaikai; Liu, Yankai; Ding, Peiyang; Qi, Yanhua; Zhang, Gaiping
• Is Part Of: Food chemistry. Volume 366(2022)
• Publication Date: 2022-01-01
• Subjects: Tiamulin -- Monoclonal antibody -- Electrochemical immunosensor -- Silver-graphene oxide nanocomposites -- AuNPs; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130573
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A murine monoclonal antibody (mAb) against tiamulin was produced. The mAb was applied to develop a label-free electrochemical immunosensor. The developed sensor exhibited wide detection range (0.01–1000 ng mL −1 ). The electrochemical immunosensor showed low detection limit (0.003 ng mL −1 ). Abstract: Based on a murine monoclonal antibody (mAb) against tiamulin (TML), an electrochemical immunosensor was proposed using silver-graphene oxide (Ag-GO) nanocomposites and gold nanocomposites (AuNPs) to detect tiamulin (TML). Due to the synergetic properties of Ag-GO nanocomposites and AuNPs, the conductivity of the immunosensor was significantly enhanced. On account of the specific mAb and conductive nanocomposites, the proposed electrochemical immunosensor exhibited a low LOD of 0.003 ng mL −1 for the detection of TML in a wide linear range of 0.01 to 1000 ng mL −1 . In addition, the immunosensor did not involve additional redox species. Furthermore, the efficient and simple electrochemical immunosensor was employed to detect TML in real samples with high accuracy, suggesting a potential detection platform for other veterinary antibiotics in animal derived foods.