Characterization of Weissella confusa DD_A7 isolated from kimchi. (August 2019)

• Record Type: Journal Article
• Authors: Dey, Debasish Kumar; Koo, Bon Gyo; Sharma, Chanchal; Kang, Sun Chul
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 111(2019)
• Page Start: 663
• Page End: 672
• Publication Date: 2019-08
• Subjects: Weissella confusa DD_A7 -- Non-pathogenic bacteria -- 16S-rRNA -- SNPs -- Antibacterial activity; NCBI National center for biotechnology information -- ML Maximum likelihood method -- MCL Maximum composite likelihood -- MRS De man Rogosa and sharpe medium -- SNP Single nucleotide polymorphism -- RMS Root mean square -- PBS Phosphate buffer saline -- BATH Bacteria adhesion to hydrocarbon -- CFCS Cell-free culture supernatant -- AO Acridine orange -- EtBr Ethidium bromide -- Ca++ Calcium -- SEM Scanning electron microscope -- SD Standard deviation -- NCCP National culture collection for pathogens -- ATCC American type culture collection; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.05.089
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Foodborne pathogenic bacteria like, Escherichia coli 0157:H7, Bacillus cereus and Salmonella typhimurium are the biggest threat to food industries. Their growth in the environment and transmission of deadly diseases has become a challenge. The present study confirmed the genetic identity of DD_A7 is similar to the reported probiotic strains of Weissella confusa by analyzing the polymorphism in the conserved region of 16S-rRNA sequence. The Kimchi isolated, non-pathogenic W. confusa DD_A7 strain has shown the potential to control the growth of foodborne pathogens. Furthermore, the cell-free culture supernatant of DD_A7 shows the potential to rapture the membrane integrity of E. coli 0157:H7 by influencing the Ca ++ ion channels. The safety concern associated with the usage of DD_A7 strain was also determined by testing its antibiotic susceptibility against different antibiotics and confirmed its non-toxic behavior on mammalian cells. Nevertheless, the strain has also encompassed several applications such as isolation of amylolytic and proteolytic enzymes. Collectively, the study will help to control the growth of foodborne pathogenic bacteria using the studied probiotic strain. In addition, the study will also help to construct a species-specific probe in future for the identification of the non-pathogenic strain of W. confusa for its application in food industries. Highlights: Weissella confusa DD_A7 is a non-pathogenic microorganism isolated from Kimchi. Result of SNP analysis revealed transversion as the most commonly occurred polymorphism. The CFCS of DD_A7 possess the antibacterial potential against some foodborne pathogens. DD_A7 has the ability of colonization in the gastrointestinal tract and it is non-toxic for the host. The strain has the amylolytic and proteolytic activity.