Fucose as a Cleavage Product of 2'Fucosyllactose Does Not Cross the Blood‐Brain Barrier in Mice. Issue 16 (5th July 2021)

• Record Type: Journal Article
• Authors: Rudloff, Silvia; Kuntz, Sabine; Borsch, Christian; Vazquez, Enrique; Buck, Rachael; Reutzel, Martina; Eckert, Gunter Peter; Kunz, Clemens
• Is Part Of: Molecular nutrition & food research. Volume 65:Issue 16(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-05
• Subjects: 13C‐labeled fucose -- biological fluids -- brain -- metabolism -- microbiota; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202100045
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: To further examine the role of the human milk oligosaccharide 2'fucosyllactose (2´FL) and fucose (Fuc) in cognition. Using 13 C‐labeled 2'FL, thestudy previously showed in mice that 13 C‐enrichment of the brain is not caused by 13 C1 ‐2´FL itself, but rather by microbial metabolites. Here, the study applies 13 C1 ‐Fuc in the same mouse model to investigate its uptake into the brain. Methods and Results: Mice received 13 C1 ‐Fuc via oral gavage (2 mmol 13 C1 ‐Fuc/kg ‐1 body weight) or intravenously (0.4 mmol/kg ‐1 body weight). 13 C‐enrichment is measured in organs, including various brain regions, biological fluids and excrements. By EA‐IRMS, the study observes an early rise of 13 C‐enrichment in plasma, 30 min after oral dosing. However, 13 C‐enrichment in the brain does not occur until 3‐5 h post‐dosing, when the 13 C‐Fuc bolus has already reached the lower gut. Therefore, the researcher assume that 13 C‐Fuc is absorbed in the upper small intestine but cannot cross the blood‐brain barrier which is also observed after intravenous application of 13 C1 ‐Fuc. Conclusions: Late 13 C‐enrichment in the rodent brain may be derived from 13 C1 ‐Fuc metabolites derived from bacterial fermentation. The precise role that Fuc or 2´FL metabolites might play in gut‐brain communication needs to be investigated in further studies. Abstract : Fucose (Fuc) is part of many human milk oligosaccharides. The study applies 13 C‐labelled Fuc to mice and measure 13 C‐enrichment in organs, different brain regions, biological fluids and excrements. Brain 13 C‐enrichment does not occur until 3‐5 h post‐dosing, when the oral 13 C‐Fuc bolus has already reached the lower gut. The study further shows that 13 C‐Fuc cannot cross the blood‐brain barrier.