Camellia nitidissima Chi leaf as pancreatic lipase inhibitors: Inhibition potentials and mechanism. Issue 9 (6th July 2021)

• Record Type: Journal Article
• Authors: Chen, Jiahui; Wu, Xuehui; Zhou, Yue; He, Junhua
• Is Part Of: Journal of food biochemistry. Volume 45:Issue 9(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-06
• Subjects: Camellia nitidissima Chi leaf -- circular dichroism -- fluorescence -- inhibition -- kinetic -- pancreatic lipase; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.13837
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: In this study, Camellia nitidissima Chi leaf extract was investigated for its compounds and pancreatic lipase inhibitory potentials. The interaction was determined using ultraviolet (UV) spectroscopy, circular dichroism (CD), fluorescence spectroscopy (FS), and molecular docking to understand the inhibiton, kinetic, and conformation of extraction–pancreatic lipase complex. C. nitidissima Chi leaf extraction inhibited the pancreatic lipase activity in a dose‐dependent manner at the concentration of 1–12 mg/ml. The Lineweaver–Burk plots indicated that the inhibition on pancreatic lipase by extraction was noncompetitive. In addition, the decrease in α ‐helix contents, increase in β‐sheet and β‐turn, and decrease in fluorescence intensity after extraction treatment indicated that the conformation of pancreatic lipase was changed. This work revealed that C. nitidissima Chi leaf extraction played a significant role in inhibiting pancreatic lipase activity and brought out a solution of delay fat accumulation. Practical applications: This study reports the components in the extract of C. nitidissima Chi leaf and its inhibitory effect and mechanism of pancreatic lipase. C. nitidissima Chi leaf is a good source of bioactive components, including multiflorin B, kaempferol‐3‐O‐rutinoside, vicenin‐2, apigenin‐6‐C‐pentosyl‐8‐C‐hexosyl, vitexin, kaempferol, and other ingredients. It can inhibit pancreatic lipase and be used to control obesity and treat hyperlipidemia. This study also revealed the structure changes of C. nitidissima Chi leaf extract on pancreatic lipase, and further revealed the inhibitory mechanism of C. nitidissima Chi leaf extract on lipase, which provides a theoretical basis for C. nitidissima Chi leaf as a lipase inhibitor. Abstract : The ingredients of Camellia nitidissima Chi leaf extraction were identified via UHPLC–MS/MS. The Camellia nitidissima Chi leaf extraction inhibit pancreatic lipase. The binding of Camellia nitidissima Chi leaf extraction to pancreatic lipase can induce secondary and tertiary conformational changes to pancreatic lipase.