Study of lincomycin partition in a recyclable thermo-pH responsive aqueous two-phase system. (October 2021)

• Record Type: Journal Article
• Authors: Wang, Yan; Yang, Ting; Zeng, Hainan; Wan, Junfen; Cao, Xuejun
• Is Part Of: Process biochemistry. Volume 109(2021)
• Page Start: 27
• Page End: 36
• Publication Date: 2021-10
• Subjects: Aqueous two-phase systems -- Licomycin -- pH-responsive polymer -- Thermo-responsive polymer; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.06.019
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Recyclable aqueous two-phase systems were constructed using EO30 PO70 and PADB5.22 . Phase formation mechanism have been studied using Low-field NMR. Lincomycin was better partitioned with partition coefficient of 5.94. The recoveries of EO30 PO70 and PADB5.22 reached 95.51 % and 96.18 %, respectively. Abstract: Aqueous two-phase systems (ATPS) have been widely used in the laboratory separation and purification of antibiotics. However, its large-scale application is limited due to the difficulty in the recovery of the phase-forming polymers. In this study, we aimed to develop a thermo-pH responsive recyclable ATPS using thermo-responsive polymer EO30 PO70 and pH-responsive polymer PADB5.22 for lincomycin partition. This ATPS can rapidly form two-phase interface in less than 5 min and can sustainably be reused. The phase separation mechanism was studied by low-field nuclear magnetic resonance (LF-NMR) and surface tension. The effects of polymer concentration, lincomycin content, pH, temperature and salts type and concentrations on lincomycin partition were investigated. The 25 % (w/v) EO30 PO70 /4.5 % (w/v) PADB5.22 ATPS showed good partitioning at room temperature for lincomycin fermentation broth in the presence of 80 mM LiCl at pH 9.1, with a partition coefficient of 5.94 and extraction recovery of 94.68 %. In addition, the recycling of the two polymers showed a high efficiency, with 95.51 % and 96.18 % recoveries of EO30 PO70 and PADB5.22 after four cycles, respectively. This recyclable ATPS has a promising industrial application in the separation and purification of antibiotics.