Detoxification of aflatoxin B1 by H2SO3 during maize wet processing, and toxicity assessment of the transformation product of aflatoxin B1. (January 2022)

• Record Type: Journal Article
• Authors: Yang, Bolei; Li, Li; Geng, Hairong; Wang, Gang; Zhang, Chenxi; Yang, Shuo; Zhao, Yueju; Xing, Fuguo; Liu, Yang
• Is Part Of: Food control. Volume 131(2022)
• Publication Date: 2022-01
• Subjects: Maize -- Aflatoxin B1 -- H2SO3 -- Detoxification -- Toxicity; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2021.108444
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Maize is susceptible to contamination with aflatoxins (AFs). Consumption of AFs contaminated maize can cause poisoning and even death. The goals of this study were to determine the detoxification efficacy of sulfurous acid (H2 SO3 ) on aflatoxin B1 (AFB1 ) within the range of soaking parameters used in industrial maize production, to identify the transformation product of AFB1 and analyze the cytotoxicity of transformation product. AFB1 contaminated maize was soaked in different concentrations of H2 SO3 and the results indicated that soaking time and temperature could significantly influence the detoxification efficiency; 25–31 μg/kg of AFB1 in maize could be detoxified by 0.2–0.3 % of H2 SO3 . The transformation product was obtained using mass spectrometry and molecular formula analysis. It was identified as C17 H14 O9 S, named as AFB1 -HSO3 . The toxicity of AFB1 -HSO3 was evaluated. Cell morphology showed that the damage caused by AFB1 was significantly greater than that caused by AFB1 -HSO3 . It also found that the effects of AFB1 -HSO3 on the activity, ATP and DNA content of Hep G2 cells were significantly less than that of AFB1 . It was concluded that AFB1 -HSO3 was less toxic than AFB1 in this system. These results suggest that H2 SO3 can be used as a potential detoxification agent in industrial maize production. Highlights: AFB1 in the contaminated maize could be detoxified by H2 SO3 . Soaking temperature and time could affect the detoxification efficiency of AFB1 . The transformation product of AFB1 was identified by LC-MS/MS as C17 H14 O9 S. AFB1 -HSO3 had lower effect on activity, ATP and DNA content of Hep G2 than AFB1 . Compared with AFB1, the cytotoxicity of AFB1 -HSO3 was significantly reduced.