Curcumin-loaded high internal phase emulsions stabilized with lysine modified lignin: a biological agent with high photothermal protection and antibacterial properties. Issue 16 (1st July 2021)

• Record Type: Journal Article
• Authors: Chen, Kai; Yuan, Shengrong; Wang, Dan; Qi, Dongming; Chen, Fengfeng; Qiu, Xueqing
• Is Part Of: Food & function. Volume 12:Issue 16(2021)
• Page Start: 7469
• Page End: 7479
• Publication Date: 2021-07-01
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo00128k
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : The UV-blocking and antioxidant properties of lysine modified enzymatic hydrolysis lignins (EHL-Lys) based high internal phase emulsions (HIPEs) for curcumin and their synergistic antibacterial activity were investigated. Abstract : Bacterial infections and multidrug resistance can seriously endanger the health and lives of humans, therefore the development of novel and efficient antibacterial strategies and drugs is urgently needed. Herein, a series of highly biocompatible lysine modified enzymatic hydrolysis lignins (EHL-Lys- x ) were synthesized using the Mannich reaction. The sterilizing efficiency of EHL-Lys-2.0 against S. aureus and E. coli at 20 mg mL −1 is 93% and 50%, respectively, which is 26% higher than pure EHL. Quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM) analysis showed that the adsorption and adhesive force between EHL-Lys- x and bacteria increase with the increased amount of grafting of Lys on EHL owing to the increase of the electrostatic interaction between the EHL-Lys- x and bacteria, which results in an improvement in the antimicrobial activity of EHL-Lys- x . Subsequently, EHL-Lys- x combined with alkyl polyglucoside (APG) was used to stabilize the high internal phase emulsion containing curcumin (HIPEs-cur). The dispersed phase fraction of HIPE-cur is 87 vol%, which is the highest internal phase reported to date in the medical research area. The highest residual levels of curcumin in HIPEs are 60-fold, 3-fold and 5-fold compared to that in bulk oil after treatment with UV radiation, thermal emittance and after storage, respectively. The minimum inhibitory concentrations of HIPEs-cur against S. aureus and E. coli were found to be 1.56 and 6.25 mg mL −1, respectively, which are far higher than that of pure EHL-Lys- x . This strategy not only increases the chemical stability and bioavailability of curcumin, but also provides a novel method for the application of lignin in biomedical science.