Transcriptome analysis of suspended aggregates formed by Listeria monocytogenes co-cultured with Ralstonia insidiosa. (December 2021)

• Record Type: Journal Article
• Authors: Chen, Qian; Li, Qun; Guo, Ailing; Liu, Ling; Gu, Lihong; Liu, Wukang; Zhang, Xinshuai; Ruan, Yao
• Is Part Of: Food control. Volume 130(2021)
• Publication Date: 2021-12
• Subjects: Listeria monocytogenes -- Ralstonia insidiosa -- Suspended aggregates -- Formation mechanism -- RNA-seq; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2021.108237
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: The resistance of food-borne pathogens to many unfavorable environments after the formation of microbial aggregates is significantly enhanced, which is an important reason for the continuous pathogen contamination. Ralstonia insidiosa can induce the food-borne pathogens to form larger multi-species suspended aggregates, but its formation mechanism is unknown, especially from a transcriptomics point of view. In this study, the condition of forming suspended aggregates by Listeria monocytogenes was investigated, and then, Illumina RNA-seq was used to compare the transcription profiles of L. monocytogenes in floating state and suspended aggregates to identify differentially expressed genes (DEGs): 511 DEGs were identified by expression analysis, including 273 up-regulated genes and 238 down-regulated genes. The functional annotation analysis results of DEGs showed that these genes were mainly involved in two-component system, phosphotransferase system, flagellar assembly, ABC transporter, glycolysis/gluconeogenesis. This study is the first report of suspended aggregates RNA-seq, and our results are of great significance to further exploring the resistance mechanism of L. monocytogenes to adverse environment and the control of pathogenic microorganisms. Highlights: L. monocytogenes could form suspended aggregates when co-cultured with R. insidiosa. Features of GO function distribution of DEGs are displayed. The KEGG pathways involved in the formation of suspended aggregates is analyzed. Some genes involved in the formation of suspended aggregates were screened.