Biosynthesis of benzyl cinnamate using an efficient immobilized lipase entrapped in nano-molecular cages. (1st December 2021)

• Record Type: Journal Article
• Authors: Cao, Yi-Ping; Zhi, Gao-Ying; Han, Li; Chen, Queting; Zhang, Dong-Hao
• Is Part Of: Food chemistry. Volume 364(2021)
• Publication Date: 2021-12-01
• Subjects: Biosynthesis -- Magnetic microspheres surface -- Nano-molecular cages -- Lipase entrapment -- Stability; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130428
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Lipase was immobilized by entrapping into nano-molecular cages. Nano-molecular cages could immobilize lipase firmly and retain its activity. Nano-molecular cages improved the stability of lipase greatly. The entrapped lipase was applied in biosynthesis of benzyl cinnamate. The entrapped lipase showed excellent activity and re-usability in use. Abstract: To improve the performance of lipase in biosynthesis of benzyl cinnamate, a new immobilized lipase by entrapping enzyme into nano-molecular cages was designed. Consequently, the entrapped lipase showed a robust immobilization, which diminished the leakage of lipase notably in use. Moreover, the entrapped lipase exhibited higher activity (57.1 U/mg) than free lipase (50.0 U/mg), demonstrating that the native conformation of lipase was not destroyed during immobilization. Compared with the adsorbed lipase (half-life 40.7 min) and free lipase (half-life 29.8 min), the entrapped lipase (half-life 85.3 min) increased the stability by about 2–3 times. Furthermore, the entrapped lipase was applied in biosynthesis of benzyl cinnamate, where it showed excellent activity and re-usability. After 7 cycles, the yield of benzyl cinnamate catalyzed by the entrapped lipase remained 70.2%, while the yield catalyzed by the adsorbed lipase was only about 10%. These results indicated that the nano-molecular cages could inhibit denaturation of lipase and maintain its activity well.