Tooth tissue engineering: tooth decellularization for natural scaffold. (June 2016)

• Record Type: Journal Article
• Authors: de Sousa Iwamoto, Luciana Aparecida; Duailibi, Monica Talarico; Iwamoto, Gerson Yoshinobu; Juliano, Yara; Duailibi, Michel Silvio; Ossamu Tanaka, Francisco André; Duailibi, Silvio Eduardo
• Is Part Of: Future science OA. Volume 2:Number 2(2016)
• Publication Date: 2016-06
• Subjects: decellularization -- demineralization -- tissue engineering -- tissue scaffold; Medicine -- Periodicals; Biotechnology -- Periodicals; 610
• DOI: 10.4155/fsoa-2016-0016
• Languages: English
• ISSNs: 2056-5623

Abstract

Aim: Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.Methodology: Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.Conclusion: This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold. Lay abstract: Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the keys to reproducing an organ or tissue is to find the ideal scaffold – natural or synthetic – for application in regenerative medicine. It should present a compatible macro- and micro-structure to guide the behavior of seeded cells. The objective of this work is to promote a natural scaffold by using decellularizing techniques for application in tooth tissue engineering. Fifty premolar teeth were divided into five groups, with different solutions. G5 was the best method to obtain a natural scaffold.