Tooth tissue engineering: tooth decellularization for natural scaffold. (June 2016) - de Sousa Iwamoto, Luciana Aparecida
Aim: Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.Methodology: Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.Conclusion: This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold. Lay abstract: Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the keys to reproducing an organ or tissue is to find the ideal scaffold – natural or synthetic – for application in regenerative medicine. It should present a compatible macro- and micro-structure to guide the behavior of seeded cells. The objective of this work is to promote a natural scaffold by using decellularizing techniques for application in tooth tissue engineering. Fifty premolar teeth were divided into five groups, with different solutions. G5 was the best method to obtain a natural scaffold.