Occurrence of lactic acid bacteria and yeasts at species and strain level during spontaneous fermentation of mawè, a cereal dough produced in West Africa. (December 2018)

• Record Type: Journal Article
• Authors: Houngbédji, Marcel; Johansen, Pernille; Padonou, Sègla Wilfrid; Akissoé, Noël; Arneborg, Nils; Nielsen, Dennis S.; Hounhouigan, D. Joseph; Jespersen, Lene
• Is Part Of: Food microbiology. Volume 76(2018)
• Page Start: 267
• Page End: 278
• Publication Date: 2018-12
• Subjects: Mawè -- Cereal doughs -- Spontaneous fermentation -- Lactic acid bacteria -- Yeast; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2018.06.005
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Mawè is a West African spontaneous fermented cereal-based dough. Different types of mawè exist varying in type of cereal and/or production condition, with fermentations lasting 24–48 h. With the aim of obtaining a comprehensive understanding of the microbial ecology of mawè processing, a microbiological characterisation was performed for four mawè types, produced at eight sites in Benin. At the onset of the fermentations lactic acid bacteria (LAB) and yeast counts were on average 7.5 ± 1.03 and 4.8 ± 0.79 Log10 cfu/g, which increased to 9.2 ± 0.38 and 7.4 ± 0.42 Log10 cfu/g, respectively, at the end of the fermentations. LAB (n = 321) and yeasts (n = 298), isolated during the fermentations, were identified. The predominant LAB and yeast species were Lactobacillus fermentum and Pichia kudriavzevii, respectively, followed by Kluyveromyces marxianus, all present throughout the mawè fermentations. Further, microbial successions took place with Weissella confusa occurring mostly at the onset, while Pediococcus acidilactici and Saccharomyces cerevisiae were mainly associated with the end of the fermentations. Species diversity was influenced both by type of cereal and production condition. The dominating strain clusters of L . fermentum and P . kudriavzevii were ubiquitous and strain diversities were influenced by type of cereal and production site. Highlights: The microbial ecology was revealed in four types of West African spontaneous fermented mawè. Among the LAB (321) isolated from mawè Lactobacillus fermentum was predominant. Among the yeasts (298) isolated from mawè the predominant species was Pichia kudriavzevii . The species diversity was influenced by type of cereal and mawè production conditions. Ubiquitous strain clusters of L. fermentum and P. kudriavzevii were identified.