Applicability of the poultry qPCR method to detect DNA of poultry processed animal protein materials. (February 2019)

• Record Type: Journal Article
• Authors: Scholtens, Ingrid M.J.; Prins, Theo W.; Margry, Rob J.C.F.; Dahlmans, Harald; van Raamsdonk, Leo W.D.
• Is Part Of: Food control. Volume 96(2019)
• Page Start: 53
• Page End: 58
• Publication Date: 2019-02
• Subjects: Sensitivity -- Poultry -- Chicken -- Turkey -- Duck -- Geese -- Species-to-species ban -- Feed -- Processed animal proteins (PAPs) -- Transmissible spongiform encephalopathy (TSE) -- Bovine spongiform encephalopathy (BSE); Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.08.035
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: After the Bovine Spongiform Encephalitis (BSE) crisis most processed animal proteins (PAPs) were banned from use in animal feed. For the foreseen reintroduction of pork PAPs in poultry feed, and poultry PAPs in pork feed and to comply with the species-to-species ban that prohibits cannibalism, a sensitive and specific TaqMan PCR detection method for poultry DNA has been designed and published. This poultry method is able to detect DNA of chicken, turkey, duck and geese in one PCR reaction. PAPs however, are a difficult and variable matrix. Therefore, the usability of the poultry method was investigated on a range of different poultry PAPs. It was shown that the poultry detection method is capable of detecting poultry DNA in eight out of nine different poultry PAPs mixed at a 0.1% level in chicken feed. The method can also detect at least 0.1% poultry PAPs mixed in pork PAPs. These results show that the poultry method fulfils the 0.1% detection limit requirement in the EU legislation. Highlights: Commercial poultry PAPs in chicken feeds and in real-life pork PAP were tested. Sensitivity of the detection method for poultry PAP appeared to be sufficient. The detection limit was shown to be at least 0.1% poultry PAP. The method performance showed deviations for some mastermixes. The PCR method meets the requirements of the EU legislation.