Profiling glucosinolate metabolites in human urine and plasma after broccoli consumption using non-targeted and targeted metabolomic analyses. (30th March 2020)

• Record Type: Journal Article
• Authors: Sun, Jianghao; Charron, Craig S.; Novotny, Janet A.; Peng, Bing; Yu, Liangli; Chen, Pei
• Is Part Of: Food chemistry. Volume 309(2020)
• Publication Date: 2020-03-30
• Subjects: Glucosinolate -- Broccoli -- Human study -- High resolution mass spectrometry -- Metabolomics, Targeted analysis -- Glucoraphanin -- Glucobrassicin -- Methoxyl glucobrassicin; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125660
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Metabolomic analysis revealed changes in endogenous metabolites after broccoli consumption. Metabolites from methoxyl-indole glucosinolates consumption are reported for the first time. Targeted analysis HRAM-MS enabled detection of low concentrations of glucosinolate metabolites at nM level. Abstract: Broccoli is a popular brassica vegetable and its consumption may decrease the occurrence of cancer in certain populations. To gain insight into the metabolites that may induce physiological responses to broccoli intake, a non-targeted metabolomic approach and a targeted approach for analysis of glucosinolate metabolites were developed using high resolution accurate mass spectrometry. A human study was conducted in which 6 subjects consumed a single meal of 200 g of uncooked broccoli florets. The metabolomic analysis revealed changes in endogenous metabolites and a decrease in hippuric acid after broccoli consumption. Targeted analysis using high-resolution, accurate mass-mass spectrometry (HRAM-MS) enabled detection of low concentrations (nM) of glucosinolate metabolites in human urine and plasma. Glucosinolate metabolites were found in human urine (13) and plasma (8), respectively. Metabolites from methoxyl-indole glucosinolates, arising from broccoli consumption, are reported for the first time. Most glucosinolate metabolites reached their peak concentration in urine 2–4 h after consumption while, in plasma, peak maxima were achieved 2 h after intake. The results suggest that glucoraphanin metabolites (sulforaphane, sulforaphane cysteine, sulforaphane N -acetyl cysteine) and indole metabolites (ascorbigen and methoxyl ascorbigen from indole glucosinolates) may serve as marker compounds for the intake of broccoli.