Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles. (February 2020)

• Record Type: Journal Article
• Authors: Anand, K.; Rajamanikandan, R.; Selva Sharma, A.; Ilanchelian, M.; Khan, Faez Iqbal; Tiloke, C.; Katari, Naresh Kumar; Boomi, P.; Balakumar, C.; Saravanan, M.; Palanisamy, S.; Ramesh, M.; Lai, Dakun; Chuturgoon, A.A.
• Is Part Of: Process biochemistry. Volume 89(2020)
• Page Start: 98
• Page End: 109
• Publication Date: 2020-02
• Subjects: Au gold -- AuNPs gold nanoparticles -- BSA bovine serum albumin -- CCM complete culture media -- DD double distilled -- DLS dynamic light scattering -- EMEM Eagle's minimum essential medium -- Fe-Pt-NPs iron-platinum nanoparticles -- FE-SEM field emission scanning electron microscopy -- FT-IR fourier transform-infrared -- GA genetic algorithm -- HEK human embryonic kidney cells -- HSA human serum albumin -- MD molecular dynamics -- MM molecular mechanics -- NPs nanoparticles -- PDB protein data bank -- PdI polydispersity index -- RMSD root mean square deviation -- RMSF root mean square fluctuation -- TEM transmission electron microscopy -- XRD X-ray diffraction -- ZnO-NPs zinc oxide nanoparticles; Pine bark extract -- Gold nanoparticles -- Human serum albumin -- HEK293 cells -- Oleamide; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.09.036
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Abstract: Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV–vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.