Reduced Campylobacter jejuni colonization in poultry gut with bioactive phenolics. (February 2018)

• Record Type: Journal Article
• Authors: Salaheen, Serajus; Tabashsum, Zajeba; Gaspard, Stefano; Dattilio, Anthony; Tran, Thomas H.; Biswas, Debabrata
• Is Part Of: Food control. Volume 84(2018)
• Page Start: 1
• Page End: 7
• Publication Date: 2018-02
• Subjects: Pomace -- Phenolics -- Campylobacter jejuni -- Colonization -- Gene expression; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2017.07.021
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: This study was designed to determine the effects of bioactive phenolics (BPE) extracted from blackberry ( Rubus fruticosus ) and blueberry ( Vaccinium corymbosum ) byproducts/pomaces on Campylobacter jejuni colonization in broiler cecum. We raised a total of 120 Cobb-500 broiler chicks in duplicate trials up to 3 weeks to determine the effect of BPE on the reduction of colonization using a C. jejuni RM1221 marker strain in broiler cecum. We observed that, as a water supplement, 1.0 g Gallic Acid Equivalent (GAE)/L of BPE reduced C. jejuni colonization level by 1 log in three weeks old broiler cecum compared to the control group. 1 g GAE/L of BPE also resulted a complete inhibition of the C. jejuni marker strain in drinking water with a potential for reduced horizontal transfer in poultry flocks. In a separate experiment, we also raised a total of 200 Cobb-500 broilers in duplicate trials up to 6 weeks to investigate natural colonization of Campylobacter in presence of BPE and observed that 1.0 g GAE/L of BPE reduced natural colonization level of Campylobacter by 2 logs in broiler cecum as a water supplement. Relative expression of several C. jejuni stress response genes, including rod shape determining protein ( mreB ) were down-regulated in the presence of sub-lethal concentration of BPE. Prolonged exposure of C. jejuni to BPE resulted the minimum inhibitory concentration (MIC) of BPE to increase from 0.5 to 1.0 mg GAE/mL; however, after single sub-culture in BPE-free broth, the MIC value revived to 0.5 mg GAE/mL. Findings from this study reveal the high potential of berry phenolics as green antimicrobials against enteric pathogen Campylobacter and application in the reduction of pre-harvest colonization level of Campylobacter in poultry gut. Highlights: Berry extracts are not cytotoxic to cultured chicken cell line. Significantly reduced colonization of C. jejuni in chicken ceca. Altered the expression of stress related genes in C. jejuni . Completely inhibited C. jejuni in drinking water reducing horizontal transfer.