Nanocomposite packaging regulates extracellular ATP and programed cell death in edible mushroom (Flammulina velutipes). (30th March 2020)

• Record Type: Journal Article
• Authors: Shi, Chong; Wu, Yuanyue; Fang, Donglu; Ma, Ning; Mariga, Alfred Mugambi; Hu, Qiuhui; Yang, Wenjian
• Is Part Of: Food chemistry. Volume 309(2020)
• Publication Date: 2020-03-30
• Subjects: Flammulina velutipes -- Nanocomposite packaging -- Extracellular ATP -- Programmed cell death; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125702
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A nano packaging with nano-Ag, TiO2, SiO2 and attapulgite was used for F. velutipes . Nano packaging inhibited eATP increase and elevated apyrase activity. Nano packaging increased NOS activity and NO content during storage. Nano packaging stimulated the gene expression of crucial enzymes in ETS. Nano packaging delayed programmed cell death in F. velutipes . Abstract: Our previous study indicated that nanocomposite packaging material (Nano-PM) containing nano-Ag, nano-TiO2, nano-SiO2 and nanoattapulgite alleviated postharvest senescence of Flammulina velutipes by regulating respiration and energy metabolism. In this study, extracellular ATP (eATP) and programmed cell death (PCD) were employed as critical factors to further investigate the senescence mechanism of postharvest F. velutipes . Results demonstrated that Nano-PM delayed apyrase activity decrease and stimulated critical oxidative phosphorylation-related gene expression to inhibit eATP content increase, which is a crucial signaling molecule related to delaying senescence. The regulation of eATP resulted in alleviating PCD including chromosomal concentration, DNA fragmentation, Ca 2+ influx, high caspase-1 activity and cytochrome c content and leading to high cell viability. Overall, Nano-PM alleviated PCD and postharvest senescence of F. velutipes by regulating extracellular ATP.