Optimization of in vitro carbohydrate digestion by mammalian mucosal α-glucosidases and its applications to hydrolyze the various sources of starches. (February 2019)

• Record Type: Journal Article
• Authors: Shin, Hansol; Seo, Dong-Ho; Seo, Jungmin; Lamothe, Lisa M.; Yoo, Sang-Ho; Lee, Byung-Hoo
• Is Part Of: Food hydrocolloids. Volume 87(2019)
• Page Start: 470
• Page End: 476
• Publication Date: 2019-02
• Subjects: Carbohydrate quality -- Glucose generation -- Mammalian α-glucosidases -- Carbohydrate digestion model; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2018.08.033
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: Glycemic carbohydrates, such as starch and sucrose, are fully hydrolyzed into monosaccharides by mucosal α-glucosidases in the small intestine. In this study, we optimized the reaction conditions of mammalian mucosal α-glucosidases to digest various types of glycemic carbohydrates to determine their hydrolytic activity. The results clearly showed that mammalian mucosal α-glucosidases had different hydrolytic properties compared to fungal amyloglucosidase, which is commonly used in vitro assay to determine carbohydrate quality, on various sizes and α-linked carbohydrates. Furthermore, non-gelatinized and gelatinized starches from different botanical sources were differently hydrolyzed to glucose by the prepared solution of mammalian α-glucosidases and porcine pancreatic α-amylase. Thus, it is clearly suggested that the digestion of glycemic carbohydrates by the optimized reaction condition with mammalian mucosal α-glucosidases and pancreatic α-amylase can be applied to determine the carbohydrate quality and digestion properties as it is more reliable to human digestive enzymes compared to fungal or microbial α-glucosidases. Graphical abstract: Image 1 Highlights: Glycemic carbohydrate digestions by mammalian α-glucosidases were optimized. Pancreatic α-amylase assists α-glucosidase activity during carbohydrate digestion. Novel approach to determine the glycemic carbohydrate quality and digestibility.