Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad. Issue 7 (19th July 2021)

• Record Type: Journal Article
• Authors: Armand-Lefèvre, Laurence; Rondinaud, Emilie; Desvillechabrol, Dimitri; Mullaert, Jimmy; Clermont, Olivier; Petitjean, Marie; Ruppe, Etienne; Cokelaer, Thomas; Bouchier, Christiane; Tenaillon, Olivier; Ma, Laurence; Nooroya, Yasmine; Matheron, Sophie; Andremont, Antoine; Denamur, Erick; Kennedy, Sean P.
• Is Part Of: Microbial genomics. Volume 7:Issue 7(2021)
• Publication Date: 2021-07-19
• Subjects: E. coli -- ESBL -- carriage -- long-term carriage -- persistence -- whole-genome sequencing -- travel; Microbial genomics -- Periodicals; 572.8629
• DOI: 10.1099/mgen.0.000576
• Languages: English
• ISSNs: 2057-5858

Abstract

Abstract : Travel to tropical regions is associated with high risk of acquiring extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) that are typically cleared in less than 3 months following return. The conditions leading to persistent carriage that exceeds 3 months in some travellers require investigation. Whole-genome sequencing (Illumina MiSeq) was performed on the 82 ESBL-E isolates detected upon return and 1, 2, 3, 6 and 12 months later from the stools of 11 long-term (>3 months) ESBL-E carriers following travel abroad. One to five different ESBL Escherichia coli strains were detected per traveller upon return, and this diminished to one after 3 months. Long-term carriage was due to the presence of the same ESBL E. coli strain, for more than 3 months, in 9 out of 11 travellers, belonging to epidemic sequence type complexes (STc 10, 14, 38, 69, 131 and 648). The mean carriage duration of strains belonging to phylogroups B2/D/F, associated with extra-intestinal virulence, was higher than that for commensal-associated A/B1/E phylogroups (3.5 vs 0.5 months, P =0.021). Genes encoding iron capture systems ( fyuA, irp ), toxins ( senB, sat ), adhesins ( flu, daaF, afa/nfaE, pap, ecpA ) and colicin ( cjrA ) were more often present in persistent strains than in transient ones. Single-nucleotide polymorphism (SNP) analysis in persistent strains showed a maximum divergence of eight SNPs over 12 months without signs of adaptation. Genomic plasticity was observed during the follow-up with the loss or gain of mobile genetic elements such as plasmids, integrons and/or transposons that may contain resistance genes at different points in the follow-up. Long-term colonization of ESBL-E following travel is primarily due to the acquisition of E. coli strains belonging to epidemic clones and harbouring 'virulence genes', allowing good adaptation to the intestinal microbiota.